Journal: bioRxiv

Article Title: Anti-HIV Immunotoxin and Antibody-Drug Conjugate Display Both Common and Distinct Effects in Killing Target Cells

doi: 10.64898/2026.04.07.717054

Figure Lengend Snippet: A . The binding of anti-gp41 7B2 to H9/N:L4-3 cells was demonstrated by indirect immunofluorescence and flow cytometry. The gray histogram indicates binding in the presence of secondary FITC-conjugated goat anti-human IgG only, the blue histogram demonstrates fluorescence of cells first incubated with 7B2 followed by FITC-conjugated secondary antibody. B. Comparative cytotoxicity of 7B2-dgA and 7B2-PNU. Cells were incubated with the indicated concentration of CIC and CD4-IgG2 (500 ng/mL) for 72 hr. MTS dye reduction was measured during the final 3 hr of culture. C. The kinetics of apoptosis induction by the CICs was measured by the binding of fluorescent annexin V to cells at different times following treatment with CIC.

Article Snippet: Cells were washed twice in PBS and incubated with 2 μg /mL of secondary FITC-conjugated goat anti-human IgG (Novus Biologicals, Centennial CO USA) for 1 hr at room temperature in the dark, then washed a final time with PBS and resuspended in 2% paraformaldehyde.

Techniques: Binding Assay, Immunofluorescence, Flow Cytometry, Fluorescence, Incubation, Concentration Assay